Evaluation of Triton Ziehl Neelsen Technique in the Detection of Intracellular Tuberculosis bacilli in Sputum
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Sridhar A
Guntur Medical College, Govt General Hospital, Guntur-522002.
iamimenotu@gmail.com
- Sundarachary N Guntur Medical College, Govt General Hospital, Guntur-522002.
Abstract
Triton X 100 reagent is a cell membrane permeabilizing agent. Its addition to conventional Ziehl Neelsen staining has been shown to improve detection of Tuberculosis bacilli in CSF by staining bacilli inside the inflammatory cells. We evaluated its performance in sputum in the diagnosis of pulmonary tuberculosis. We compared the Triton ZN technique with the conventional ZN method on the sputum of 68 patients suspected with pulmonary tuberculosis by overall agreement analysis. The Triton ZN technique was not superior to the conventional AFB staining method at all the tested concentrations of Triton X100 by an overall percent agreement ranging from 95.4% to 72.1%, positive percent agreement 91.65% to 9.1% and negative percent agreement 96.2% to 87.5%. Our study suggests that Triton X 100 added to conventional ZN staining does not improve detection of M. tuberculosis in sputum
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